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Image Search Results
Journal: Neuron
Article Title: Sensory Experience Engages Microglia to Shape Neural Connectivity through a Non-Phagocytic Mechanism.
doi: 10.1016/j.neuron.2020.08.002
Figure Lengend Snippet: Figure 5. Microglial TWEAK Signals through Postsynaptic Fn14 to Decrease Bulbous Spine Numbers (A) Example images of spines in the dLGNs of Fn14fl/flCre-negative or VGLUT2-Cre-positive mice following viral infection. Scale bar, 2 mm. (B) Quantification of bulbous spine densities following TWEAK or mCherry expression in the dLGNs of Cre-negative () and VGLUT2-Cre-positive (+) mice. A comparison of mCherry-infected conditions is also plotted in Figure 2D. (C) Example images of spines in the dLGNs of TWEAKfl/flCre-negative or Cx3cr1-Cre-positive mice. Scale bar, 2 mm. (D) Bulbous spine density is increased by genetic ablation of TWEAK in microglia. (E) Spine head diameter is increased by genetic ablation of TWEAK in microglia. (F) Thin spine density is unaffected by genetic ablation of TWEAK in microglia. (G) Non-bulbous spine density is unaffected by genetic ablation of TWEAK in microglia. (H) Total spine density is unaffected by genetic ablation of TWEAK in microglia. (I) Spine length is unaffected by genetic ablation of TWEAK in microglia. (J) Confocal images of dLGNs from a TWEAK KO mouse, a Fn14fl/flCre-negative mouse, and a Fn14fl/fl; VGLUT2-Cre+ mouse following bath application of recombinant mouse TWEAK and subsequent immunostaining for TWEAK (red) and VGLUT2 (green). Scale bar, 10 mm. (K) Western blot of whole mouse forebrain fractionated to enrich for synaptosomes. Blots were probed for Fn14, the retinal presynaptic marker VGLUT2, the postsynaptic marker PSD-95, and GAPDH (a non-synaptic control). (legend continued on next page)
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: B6.129P2(Cg)-Cx3cr1tm1Litt/J The Jackson Laboratory 005582; RRID:IMSR_JAX:005582 Mouse: Slc17a6tm2(cre)Lowl/J The Jackson Laboratory 028863; RRID:IMSR_JAX:016963 Mouse: Tg(Prkcd-glc-1/CFP,-Cre) Haubensak et al., 2010 N/A Mouse: B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J The Jackson Laboratory 007914; RRID:IMSR_JAX:007914 Mouse: Tg(Chx10-EGFP/cre,-ALPP)2Clc/J The Jackson Laboratory 005105; RRID:IMSR_JAX:005105 Mouse: B6J.B6N(Cg)-Cx3cr1tm1.1(cre)Jung/J The Jackson Laboratory 025524; RRID:IMSR_JAX:025524 Mouse: B6.C1qa(KO) Lab of Beth Stevens N/A Oligonucleotides qPCR primer: Gapdh (Forward): GGGTGTGAACCACG AGAAATA Origene Cat #: MP205604 qPCR primer: Gapdh (Reverse): CTGTGGTCATGAG CCCTTC Origene Cat #: MP205604 qPCR primer:
Techniques: Infection, Expressing, Comparison, Recombinant, Immunostaining, Western Blot, Marker, Control
Journal: Neuron
Article Title: Sensory Experience Engages Microglia to Shape Neural Connectivity through a Non-Phagocytic Mechanism.
doi: 10.1016/j.neuron.2020.08.002
Figure Lengend Snippet: Figure 8. Model of TWEAK/Fn14-Dependent Synapse Regulation during Experience-Dependent Refinement (A) Schematic of retinal inputs (orange) converging onto the dendrites of a relay neuron (teal). Alone, Fn14 increases bulbous spines to strengthen and maintain synapses, whereas TWEAK binding at other synapses leads to their ultimate disassembly. In the absence of experience, neither TWEAK nor Fn14 is expressed, so neither of these processes occur, and synapses remain in a weakened state but are not properly removed. (B) We propose that the SD period of postsynaptic regulation by microglia identified in this study constitutes a later phase of microglia-driven circuit sculpting that follows earlier phases of phagocytic pruning and is driven by distinct molecular mechanisms.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: B6.129P2(Cg)-Cx3cr1tm1Litt/J The Jackson Laboratory 005582; RRID:IMSR_JAX:005582 Mouse: Slc17a6tm2(cre)Lowl/J The Jackson Laboratory 028863; RRID:IMSR_JAX:016963 Mouse: Tg(Prkcd-glc-1/CFP,-Cre) Haubensak et al., 2010 N/A Mouse: B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J The Jackson Laboratory 007914; RRID:IMSR_JAX:007914 Mouse: Tg(Chx10-EGFP/cre,-ALPP)2Clc/J The Jackson Laboratory 005105; RRID:IMSR_JAX:005105 Mouse: B6J.B6N(Cg)-Cx3cr1tm1.1(cre)Jung/J The Jackson Laboratory 025524; RRID:IMSR_JAX:025524 Mouse: B6.C1qa(KO) Lab of Beth Stevens N/A Oligonucleotides qPCR primer: Gapdh (Forward): GGGTGTGAACCACG AGAAATA Origene Cat #: MP205604 qPCR primer: Gapdh (Reverse): CTGTGGTCATGAG CCCTTC Origene Cat #: MP205604 qPCR primer:
Techniques: Binding Assay